Selected article for: "lysate preparation and lysis buffer"
Author: Roni Ben-Ami; Agnes Klochendler; Matan Seidel; Tal Sido; Ori Gurel-Gurevich; Moran Yassour; Eran Meshorer; Gil Benedek; Irit Fogel; Esther Oiknine-Djian; Asaf Gertler; Zeev Rotstein; Bruno Lavi; Yuval Dor; Dana G Wolf; Maayan Salton; Yotam Drier
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection
  • Document date: 2020_4_22
    • ID: 5s03f0pp_30
    Snippet: Nasopharyngeal swab samples were collected in 2 ml Viral Transport Medium (VTM) and mixed 1:1 with a 2x concentrated Zymo lysis buffer, or collected directly to 2 ml Zymo lysis buffer. For the initial validation, samples were collected from symptomatic patients or from screened healthy asymptomatic subjects. For sample lysate preparation 220 µL of sample VTM were added to 280 µL lysis buffer. RNA was extracted using MagNA Pure 96 kit (Roche Lif.....
    Document: Nasopharyngeal swab samples were collected in 2 ml Viral Transport Medium (VTM) and mixed 1:1 with a 2x concentrated Zymo lysis buffer, or collected directly to 2 ml Zymo lysis buffer. For the initial validation, samples were collected from symptomatic patients or from screened healthy asymptomatic subjects. For sample lysate preparation 220 µL of sample VTM were added to 280 µL lysis buffer. RNA was extracted using MagNA Pure 96 kit (Roche Lifesciences) using Roche platform and eluted in 60 µL. Ten µL of RNA was used in 30 µL reaction using Real-Time Fluorescent RT-PCR kit (BGI).

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