Author: Roni Ben-Ami; Agnes Klochendler; Matan Seidel; Tal Sido; Ori Gurel-Gurevich; Moran Yassour; Eran Meshorer; Gil Benedek; Irit Fogel; Esther Oiknine-Djian; Asaf Gertler; Zeev Rotstein; Bruno Lavi; Yuval Dor; Dana G Wolf; Maayan Salton; Yotam Drier
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Document date: 2020_4_22
ID: 5s03f0pp_5
Snippet: While several pooling approaches for SARS-CoV-2 detection were recently suggested (e.g. [16] [17] [18] [19] ), none demonstrated adherence to current clinical standards, nor shown to work for performing RNA extraction in pools, which is typically the limiting step. Here we describe and demonstrate practical pooling solutions that save time and reagents by performing RNA extraction and RT-PCR on pooled samples. Unlike other suggestions of large-. .....
Document: While several pooling approaches for SARS-CoV-2 detection were recently suggested (e.g. [16] [17] [18] [19] ), none demonstrated adherence to current clinical standards, nor shown to work for performing RNA extraction in pools, which is typically the limiting step. Here we describe and demonstrate practical pooling solutions that save time and reagents by performing RNA extraction and RT-PCR on pooled samples. Unlike other suggestions of large-. CC-BY-NC 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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