Author: Takako I. Jones; Guo-Liang Chew; Pamela Barraza-Flores; Spencer Schreier; Monique Ramirez; Ryan D. Wuebbles; Dean J. Burkin; Robert K. Bradley; Peter L. Jones
Title: Transgenic mice expressing tunable levels of DUX4 develop characteristic facioscapulohumeral muscular dystrophy-like pathophysiology ranging in severity Document date: 2018_11_15
ID: 1yto01tr_19
Snippet: The 10 µm cryosections of muscle tissue were fixed with 4% PFA/PBS on ice for 10 min, permeabilized with 0.25% TritonX-100/PBS for 10 min, then incubated with blocking solution (5% normal goat serum, 2% BSA, 0.01% TritonX-100/PBS) for 30 min, and then incubated with primary antibody at 4ºC overnight. The following day, sections were incubated with secondary antibody at room temperature for 40 min, then mounted in ProLong Gold with DAPI for nucl.....
Document: The 10 µm cryosections of muscle tissue were fixed with 4% PFA/PBS on ice for 10 min, permeabilized with 0.25% TritonX-100/PBS for 10 min, then incubated with blocking solution (5% normal goat serum, 2% BSA, 0.01% TritonX-100/PBS) for 30 min, and then incubated with primary antibody at 4ºC overnight. The following day, sections were incubated with secondary antibody at room temperature for 40 min, then mounted in ProLong Gold with DAPI for nuclear staining. DUX4 was detected using E5-5 antibody (Abcam ab124699) diluted 1:200.
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