Author: Lucia de Noronha; Camila Zanluca; Marion Burger; Andreia Akemi Suzukawa; Marina Azevedo; Patricia Z. Rebutini; Iolanda Maria Novadzki; Laurina Setsuko Tanabe; Mayra Marinho Presibella; Claudia Nunes Duarte dos Santos
Title: Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues Document date: 2018_7_16
ID: d28hr3fv_9
Snippet: ) for both routine hematoxylin-eosin (H&E) staining and IHC staining. Villus maturation was evaluated by considering the villus morphology and the gestational age at delivery. Morphometric analyses were performed in placental tissue specimens from mothers who were infected with ZIKV during the third trimester of pregnancy (n = 6). A negative control group (n = 5) was used for comparison (Baurakiades et al., 2011) . For each case, 30 high-power fi.....
Document: ) for both routine hematoxylin-eosin (H&E) staining and IHC staining. Villus maturation was evaluated by considering the villus morphology and the gestational age at delivery. Morphometric analyses were performed in placental tissue specimens from mothers who were infected with ZIKV during the third trimester of pregnancy (n = 6). A negative control group (n = 5) was used for comparison (Baurakiades et al., 2011) . For each case, 30 high-power fields (HPFs = 400×) were randomly selected, and the villi, CD163+ HCs, knots and sprouts in each field were counted. The mean values of these parameters over the 30 fields were used for the statistical analyses. Statistical analyses were performed using GraphPad Prism 5.03 software. The Zikainfected and uninfected control groups were compared using the Mann-Whitney test. Differences were considered to be statistically significant at p<0.05.
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