Selected article for: "previous study and study report"
Author: Changhai Lei; Wenyan Fu; Kewen Qian; Tian Li; Sheng Zhang; Min Ding; Shi Hu
Title: Potent neutralization of 2019 novel coronavirus by recombinant ACE2-Ig
  • Document date: 2020_2_2
    • ID: aeuy92bx_9
    Snippet: For recombinant fusion plasmid, DNA sequences of extracellular domains (ECDs) of ACE2 (aa: 1-740) were ligated to the Fc segment of human IgG1. Mutations were generated by integrated DNA Technologies. We use the FreeStyle 293 expression system (Invitrogen) to express the fusion proteins. Vectors and detailed methods were according to our previous study 21 . Fusion proteins were further purified using protein A Sepharose with the harvested cell cu.....
    Document: For recombinant fusion plasmid, DNA sequences of extracellular domains (ECDs) of ACE2 (aa: 1-740) were ligated to the Fc segment of human IgG1. Mutations were generated by integrated DNA Technologies. We use the FreeStyle 293 expression system (Invitrogen) to express the fusion proteins. Vectors and detailed methods were according to our previous study 21 . Fusion proteins were further purified using protein A Sepharose with the harvested cell culture supernatant. TIGIT-Ig, which were described in our pervious report 13 , served as a control in our study. The concentration and purity of the fusion protein were determined by measuring the UV absorbance at a wavelength of 280 nm and by polyacrylamide gel electrophoresis, respectively.

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