Author: Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono
Title: An interaction network of RNA-binding proteins involved in Drosophila oogenesis Document date: 2020_1_9
ID: 2f9nc2to_7
Snippet: Membranes were blocked in PBS containing 5% milk powder and 0.1% Tween-20. HA-tagged, HA-Flagtagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000, Thermo Fisher Scientific #A11122) respectively. Blots were developed with ECL (GE healthcare) reagents, as recommended by the manufacturer, and imaged using an Amersham Imager 600 (GE healthcare)......
Document: Membranes were blocked in PBS containing 5% milk powder and 0.1% Tween-20. HA-tagged, HA-Flagtagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000, Thermo Fisher Scientific #A11122) respectively. Blots were developed with ECL (GE healthcare) reagents, as recommended by the manufacturer, and imaged using an Amersham Imager 600 (GE healthcare).
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