Author: Lucia de Noronha; Camila Zanluca; Marion Burger; Andreia Akemi Suzukawa; Marina Azevedo; Patricia Z. Rebutini; Iolanda Maria Novadzki; Laurina Setsuko Tanabe; Mayra Marinho Presibella; Claudia Nunes Duarte dos Santos
Title: Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues Document date: 2018_7_16
ID: d28hr3fv_5
Snippet: Anti-ZIKV IgM in serum samples was detected with an in-house IgM capture enzymelinked immunosorbent assay (MAC-ELISA) using β -propiolactone-inactivated ZIKV and MOCK (from noninfected cells) cell cultures as antigens according to the Centers for Disease Control and Prevention (CDC. Centers for Disease Control and Prevention, 2017) guidelines, with minor modifications. A humanized anti-flavivirus monoclonal antibody (MAb) was kindly provided by .....
Document: Anti-ZIKV IgM in serum samples was detected with an in-house IgM capture enzymelinked immunosorbent assay (MAC-ELISA) using β -propiolactone-inactivated ZIKV and MOCK (from noninfected cells) cell cultures as antigens according to the Centers for Disease Control and Prevention (CDC. Centers for Disease Control and Prevention, 2017) guidelines, with minor modifications. A humanized anti-flavivirus monoclonal antibody (MAb) was kindly provided by the CDC and was used as a positive control. Serum samples were also tested for anti-DENV antibodies using either the Panbio Dengue IgM Capture ELISA or the Panbio Dengue IgG Indirect ELISA (Alere, Brisbane, Australia) following the manufacturer's instructions.
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