Author: Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu
Title: Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM Document date: 2020_3_5
ID: cr22vp8b_32
Snippet: Frozen grid was transferred to a Titan Krios transmission electron microscope (Thermo Fisher Scientific) performed at the 300 kV and images were recorded by a Falcon III direct electron detector (Thermo Fisher Scientific). Image stacks with 16 frames were collected in linear mode by EPU at a nominal magnification of 59000 x, corresponding to a pixel size of 1.14 Ã…/pixel. Total dose was estimated at about 60 e -/Ã… 2 . Length, the diameter of ste.....
Document: Frozen grid was transferred to a Titan Krios transmission electron microscope (Thermo Fisher Scientific) performed at the 300 kV and images were recorded by a Falcon III direct electron detector (Thermo Fisher Scientific). Image stacks with 16 frames were collected in linear mode by EPU at a nominal magnification of 59000 x, corresponding to a pixel size of 1.14 Ã…/pixel. Total dose was estimated at about 60 e -/Ã… 2 . Length, the diameter of stem and spike's head are 23nm, 4nm and 7nm, respectively.
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