Selected article for: "primer set and SARS detect"
Author: Reynés, Bàrbara; Serra, Francisca; Palou, Andreu
Title: Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification
  • Cord-id: 4oq2cr8g
  • Document date: 2021_4_6
    • ID: 4oq2cr8g
    Snippet: Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are p
    Document: Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection.

    Search related documents:
    Co phrase search for related documents
    • accurate testing and active infection: 1, 2, 3
    • accurate testing and acute infection: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • accurate testing and low number: 1, 2, 3
    • acid amplification test and active infection: 1
    • acid amplification test and acute infection: 1, 2, 3, 4, 5, 6, 7
    • acid amplification test and low concentration: 1
    • active infection and acute infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • active infection and low concentration: 1
    • active infection and low number: 1, 2, 3
    • active infection presence and acute infection: 1
    • active sars infection and acute infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • active sars infection and low concentration: 1
    • active sars infection and low number: 1
    • acute infection and low concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • acute infection and low number: 1, 2, 3, 4, 5, 6, 7, 8, 9