Author: Paul A. Rowley; Brandon Ho; Sarah Bushong; Arlen Johnson; Sara L. Sawyer
Title: XRN1 is a Species-Specific Virus Restriction Factor in Yeasts Document date: 2016_8_16
ID: 1znuxoj5_29
Snippet: kudriavzevii and converted to cDNA using random hexamer priming. cDNA samples 513 were amplified using primers designed to specifically target SkV-L-A1 GAG and the 514 cellular gene TAF10. The empty vector control was used as the calibrator sample, and 515 TAF10 expression was used as the normalizer to calculate the relative amount of SkV-L-516 A1 RNAs present within each XRN1 expressing S. kudriavzevii cell line using the 517 comparative CT meth.....
Document: kudriavzevii and converted to cDNA using random hexamer priming. cDNA samples 513 were amplified using primers designed to specifically target SkV-L-A1 GAG and the 514 cellular gene TAF10. The empty vector control was used as the calibrator sample, and 515 TAF10 expression was used as the normalizer to calculate the relative amount of SkV-L-516 A1 RNAs present within each XRN1 expressing S. kudriavzevii cell line using the 517 comparative CT method [65] . We found that expression of XRN1 from S. kudriavzevii (n 518 = 10) reduced the relative levels of SkV-L-A1 RNAs by 40% (Fig 7E) , even though this
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