Author: Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu
Title: Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM Document date: 2020_3_5
ID: cr22vp8b_24
Snippet: Virus was amplified in Vero cell in an incubator at 37°C and 5% CO 2 for 96 hours and the viral load was determined by qPCR assay. The harvested 400ml virus supernatant with a virus titer of about 1.08 × 10 5 TCID 50 /ml (genome equivalent) was firstly inactivated by adding 0.05% (v/v) β-propiolactone and incubating at 4℃ for 36 hours......
Document: Virus was amplified in Vero cell in an incubator at 37°C and 5% CO 2 for 96 hours and the viral load was determined by qPCR assay. The harvested 400ml virus supernatant with a virus titer of about 1.08 × 10 5 TCID 50 /ml (genome equivalent) was firstly inactivated by adding 0.05% (v/v) β-propiolactone and incubating at 4℃ for 36 hours.
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