Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_29
Snippet: • After a quick spin (<500 x g), place the tubes on a magnet stand to clear and withdraw the liquid with the pipettor set to 5 µL less than the volume to be removed. • After a quick spin, replace on the magnet stand and withdraw the last drop with a 20 µL pipette tip. • With the tubes still on the magnet stand, carefully add 500 µL Wash buffer. The surface tension will cause the beads to slide up along the side of the tube closest to the.....
Document: • After a quick spin (<500 x g), place the tubes on a magnet stand to clear and withdraw the liquid with the pipettor set to 5 µL less than the volume to be removed. • After a quick spin, replace on the magnet stand and withdraw the last drop with a 20 µL pipette tip. • With the tubes still on the magnet stand, carefully add 500 µL Wash buffer. The surface tension will cause the beads to slide up along the side of the tube closest to the magnet. • Slowly withdraw the liquid with a 1 mL pipette tip without disturbing the beads.
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