Viral fitness predicts the magnitude and direction of perturbations in the infected host transcriptome

Héctor Cervera; Silvia Ambrós; Guillermo P. Bernet; Guillermo Rodrigo; Santiago F. Elena

Validation of gene expression through RT-qPCR. Total RNA was extracted from 100 mg of fresh tissue of plants infected with each one of the seven TEV genotypes as described above. The concentration of total plant RNA was adjusted to 50 ng/µL. Nine candidate genes were selected to validate the effect of infection with each TEV genotype on their expression. Specific primers were designed for each gene that amplified the matured version of their corresponding mRNAs. Primers were designed using OLIGO Primer Analysis Software version 7 (www.oligo.net). The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/206789 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/206789 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/206789 doi: bioRxiv preprint