Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection Document date: 2020_1_16
ID: 8kced06y_31
Snippet: For the detection of viral RNA extracted from urine, we first spiked the ZIKV RNA with blocking oligos (200 nM) into 200 µl human urine to mimic the clinic sample. Then, we used Quick-RNA Viral Kit (Zymo research) to extract the viral RNA from it. Different amount of ZIKV RNA were tested (Fig. 4A) ......
Document: For the detection of viral RNA extracted from urine, we first spiked the ZIKV RNA with blocking oligos (200 nM) into 200 µl human urine to mimic the clinic sample. Then, we used Quick-RNA Viral Kit (Zymo research) to extract the viral RNA from it. Different amount of ZIKV RNA were tested (Fig. 4A) .
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