Author: Yasunori Watanabe; Zachary T. Berndsen; Jayna Raghwani; Gemma E. Seabright; Joel D. Allen; Jason S. McLellan; Ian A. Wilson; Thomas A. Bowden; Andrew B. Ward; Max Crispin
Title: Vulnerabilities in coronavirus glycan shields despite extensive glycosylation Document date: 2020_2_21
ID: bnnt05fn_31
Snippet: Human embryonic kidney 293 Freestyle (HEK293F) cells were transfected with mammaliancodon-optimised genes encoding 2P-stabilised SARS MERS and HKU1 S proteins, as previously described 41 . H3N2 Victoria 2011 hemagglutinin was also expressed in the HEK 293F cells. Cultures were harvested 6 days after transfection, filtered and purified by nickelaffinity chromatography and size exclusion chromatography using a Superdex TM 16/600 75 pg column (GE He.....
Document: Human embryonic kidney 293 Freestyle (HEK293F) cells were transfected with mammaliancodon-optimised genes encoding 2P-stabilised SARS MERS and HKU1 S proteins, as previously described 41 . H3N2 Victoria 2011 hemagglutinin was also expressed in the HEK 293F cells. Cultures were harvested 6 days after transfection, filtered and purified by nickelaffinity chromatography and size exclusion chromatography using a Superdex TM 16/600 75 pg column (GE Healthcare).
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