Selected article for: "publication submit decision and study design"

Author: Fafi-Kremer, Samira; Bruel, Timothée; Madec, Yoann; Grant, Rebecca; Tondeur, Laura; Grzelak, Ludivine; Staropoli, Isabelle; Anna, François; Souque, Philippe; Fernandes-Pellerin, Sandrine; Jolly, Nathalie; Renaudat, Charlotte; Ungeheuer, Marie-Noëlle; Schmidt-Mutter, Catherine; Collongues, Nicolas; Bolle, Alexandre; Velay, Aurélie; Lefebvre, Nicolas; Mielcarek, Marie; Meyer, Nicolas; Rey, David; Charneau, Pierre; Hoen, Bruno; De Seze, Jérôme; Schwartz, Olivier; Fontanet, Arnaud
Title: Serologic responses to SARS-CoV-2 infection among hospital staff with mild disease in eastern France
  • Cord-id: a9cesbuv
  • Document date: 2020_7_31
  • ID: a9cesbuv
    Snippet: BACKGROUND: The serologic response of individuals with mild forms of SARS-CoV-2 infection is poorly characterized. METHODS: Hospital staff who had recovered from mild forms of PCR-confirmed SARS-CoV-2 infection were tested for anti-SARS-CoV-2 antibodies using two assays: a rapid immunodiagnostic test (99.4% specificity) and the S-Flow assay (~99% specificity). The neutralizing activity of the sera was tested with a pseudovirus-based assay. FINDINGS: Of 162 hospital staff who participated in the
    Document: BACKGROUND: The serologic response of individuals with mild forms of SARS-CoV-2 infection is poorly characterized. METHODS: Hospital staff who had recovered from mild forms of PCR-confirmed SARS-CoV-2 infection were tested for anti-SARS-CoV-2 antibodies using two assays: a rapid immunodiagnostic test (99.4% specificity) and the S-Flow assay (~99% specificity). The neutralizing activity of the sera was tested with a pseudovirus-based assay. FINDINGS: Of 162 hospital staff who participated in the investigation, 160 reported SARS-CoV-2 infection that had not required hospital admission and were included in these analyses. The median time from symptom onset to blood sample collection was 24 days (IQR: 21–28, range 13–39). The rapid immunodiagnostic test detected antibodies in 153 (95.6%) of the samples and the S-Flow assay in 159 (99.4%), failing to detect antibodies in one sample collected 18 days after symptom onset (the rapid test did not detect antibodies in that patient). Neutralizing antibodies (NAbs) were detected in 79%, 92% and 98% of samples collected 13–20, 21–27 and 28–41 days after symptom onset, respectively (P = 0.02). INTERPRETATION: Antibodies against SARS-CoV-2 were detected in virtually all hospital staff sampled from 13 days after the onset of COVID-19 symptoms. This finding supports the use of serologic testing for the diagnosis of individuals who have recovered from SARS-CoV-2 infection. The neutralizing activity of the antibodies increased overtime. Future studies will help assess the persistence of the humoral response and its associated neutralization capacity in recovered patients. FUNDINGS: The funders had no role in study design, data collection, interpretation, or the decision to submit the work for publication.

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