Author: MS Zinter; CC Dvorak; MY Mayday; K Iwanaga; NP Ly; ME McGarry; GD Church; LE Faricy; CM Rowan; JR Hume; ME Steiner; ED Crawford; C Langelier; K Kalantar; ED Chow; S Miller; K Shimano; A Melton; GA Yanik; A Sapru; JL DeRisi
Title: Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children Document date: 2018_3_29
ID: 28nlawnb_36
Snippet: Our study has several strengths. First, we optimized the identification of Aspergillus RNA and contextualized Aspergillus sequencing reads by comparing with both growth in culture and BAL galactomannan. Second, we proposed a logical analytical framework that ranks organism abundance both within a sample and relative to other samples; this framework allowed us to rapidly exclude more than 95% of identified bacteria. Third, we provide, to our knowl.....
Document: Our study has several strengths. First, we optimized the identification of Aspergillus RNA and contextualized Aspergillus sequencing reads by comparing with both growth in culture and BAL galactomannan. Second, we proposed a logical analytical framework that ranks organism abundance both within a sample and relative to other samples; this framework allowed us to rapidly exclude more than 95% of identified bacteria. Third, we provide, to our knowledge, the first evaluation of the pulmonary microbiome in immunocompromised children.
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