Author: MS Zinter; CC Dvorak; MY Mayday; K Iwanaga; NP Ly; ME McGarry; GD Church; LE Faricy; CM Rowan; JR Hume; ME Steiner; ED Crawford; C Langelier; K Kalantar; ED Chow; S Miller; K Shimano; A Melton; GA Yanik; A Sapru; JL DeRisi
Title: Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children Document date: 2018_3_29
ID: 28nlawnb_6
Snippet: Our laboratory has previously characterized high-throughput mNGS protocols for use with human cerebrospinal and intravitreal fluid (9) (10) (11) . Given the challenges of diagnosing pulmonary infections, we created a set of mock-positive BALs by spiking a pre-determined number of colony forming units (CFUs) of an Aspergillus niger clinical isolate into aliquots of BAL with a previously identified Haemophilus influenzae/Human Adenovirus B co-infec.....
Document: Our laboratory has previously characterized high-throughput mNGS protocols for use with human cerebrospinal and intravitreal fluid (9) (10) (11) . Given the challenges of diagnosing pulmonary infections, we created a set of mock-positive BALs by spiking a pre-determined number of colony forming units (CFUs) of an Aspergillus niger clinical isolate into aliquots of BAL with a previously identified Haemophilus influenzae/Human Adenovirus B co-infection (Supplemental Text 1).
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