Selected article for: "cDNA synthesis and polymerase chain reaction"
Author: Anna R. Sjodin; Michael R. Willig; Simon J. Anthony
Title: Quantitative Delineation of Herpesviruses in Bats for use in Ecological Studies
  • Document date: 2019_11_26
    • ID: 21ldw4lk_10
    Snippet: Viral Screening: Total nucleic acids were extracted from each swab using the EasyMag platform (bioMerieux, Inc.), and cDNA was synthesized using SuperScript III first-strand synthesis supermix (Invitrogen). Nested consensus polymerase chain reaction (cPCR) was performed twice on each sample, targeting a region of the highly conserved catalytic subunit of the DNA polymerase gene (Van deVanter et al. 1996) . cPCR is a broadly reactive method that a.....
    Document: Viral Screening: Total nucleic acids were extracted from each swab using the EasyMag platform (bioMerieux, Inc.), and cDNA was synthesized using SuperScript III first-strand synthesis supermix (Invitrogen). Nested consensus polymerase chain reaction (cPCR) was performed twice on each sample, targeting a region of the highly conserved catalytic subunit of the DNA polymerase gene (Van deVanter et al. 1996) . cPCR is a broadly reactive method that allows detection of novel viruses by using nonspecific primers targeted at viral families or genera (Anthony et al. 2013 (Anthony et al. , 2015 . A constructed synthetic plasmid was used as a positive control to confirm effective implementation of the assay and to detect possible contamination.

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