Author: Zeng, Chunxi; Hou, Xucheng; Yan, Jingyue; Zhang, Chengxiang; Li, Wenqing; Zhao, Weiyu; Du, Shi; Dong, Yizhou
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Cord-id: aju2nr9x Document date: 2020_4_5
ID: aju2nr9x
Snippet: SARS-CoV-2 has rapidly become a pandemic worldwide; therefore, an effective vaccine is urgently needed. Recently, messenger RNAs (mRNAs) have emerged as a promising platform for vaccination. Here, we systematically investigated the untranslated regions (UTRs) of mRNAs in order to enhance protein production. Through a comprehensive analysis of endogenous gene expression and de novo design of UTRs, we identified the optimal combination of 5’ and 3’ UTR, termed as NASAR, which was five to ten-f
Document: SARS-CoV-2 has rapidly become a pandemic worldwide; therefore, an effective vaccine is urgently needed. Recently, messenger RNAs (mRNAs) have emerged as a promising platform for vaccination. Here, we systematically investigated the untranslated regions (UTRs) of mRNAs in order to enhance protein production. Through a comprehensive analysis of endogenous gene expression and de novo design of UTRs, we identified the optimal combination of 5’ and 3’ UTR, termed as NASAR, which was five to ten-fold more efficient than the tested endogenous UTRs. More importantly, NASAR mRNAs delivered by lipid-derived nanoparticles showed dramatic expression of potential SARS-CoV-2 antigens both in vitro and in vivo. These NASAR mRNAs merit further development as alternative SARS-CoV-2 vaccines.
Search related documents:
Co phrase search for related documents- aat antitrypsin and luciferase expression: 1
- aat antitrypsin tf transferrin mrnas and luciferase expression: 1
- additional mrnas and luciferase expression: 1, 2
- additional mrnas and luciferase expression result: 1
Co phrase search for related documents, hyperlinks ordered by date