Selected article for: "bacterial load and rRNA gene"

Author: Osman, Shariff; La Duc, Myron T; Dekas, Anne; Newcombe, David; Venkateswaran, Kasthuri
Title: Microbial burden and diversity of commercial airline cabin air during short and long durations of travel
  • Cord-id: akt57fa6
  • Document date: 2008_2_7
  • ID: akt57fa6
    Snippet: Total microbial burden and diversity associated with commercial airliner cabin air was assessed by molecular methods in 125 air samples from the business-class sections of 16 domestic and international flights. Viable microbial burden within these cabin air parcels constituted only 1–10% of the total microbial population and ranged from below detection limits to 1.2 × 10(4) cells m(–3) as determined with a validated ATP-based technology. Cultivable bacterial diversity was almost entirely li
    Document: Total microbial burden and diversity associated with commercial airliner cabin air was assessed by molecular methods in 125 air samples from the business-class sections of 16 domestic and international flights. Viable microbial burden within these cabin air parcels constituted only 1–10% of the total microbial population and ranged from below detection limits to 1.2 × 10(4) cells m(–3) as determined with a validated ATP-based technology. Cultivable bacterial diversity was almost entirely limited to Gram-positive bacteria such as Staphylococcus and Bacillus. In contrast, cloning and sequencing 16S rRNA gene directly from the samples without cultivation indicated a significantly broader diversity, as sequences representing more than 100 species, and encompassing 12 classes of bacteria, were retrieved in varying abundance. Sequences of proteobacterial and Gram-positive lineage were retrieved most frequently (58% and 31% of all clone sequences, respectively), with Gram-positive and α-proteobacterial sequences dominating international flight samples and β- and γ-proteobacterial sequences comprising the largest portion of those retrieved from domestic flights. Significant differences in bacterial load and diversity were noted between samples obtained on domestic and international flights. The disparities observed in microbial abundance and diversity further underscore the immense value of state-of-the art molecular assays in augmenting traditional culture-based techniques. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/ismej.2008.11) contains supplementary material, which is available to authorized users.

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