Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_57
Snippet: This study describes a sequencing approach for rapid and accurate identification of IBV genotypes/lineages utilizing the MinION Nanopore sequencer. Additionally, a sequence analysis workflow is described here. The workflow analysis is designed to deal with the multiple, highly similar sequences contained within GenBank that are due to numerous sequencing experiments of IBV (especially vaccine viruses). This protocol demonstrates the feasibility o.....
Document: This study describes a sequencing approach for rapid and accurate identification of IBV genotypes/lineages utilizing the MinION Nanopore sequencer. Additionally, a sequence analysis workflow is described here. The workflow analysis is designed to deal with the multiple, highly similar sequences contained within GenBank that are due to numerous sequencing experiments of IBV (especially vaccine viruses). This protocol demonstrates the feasibility of real-time-based sequencing to provide isolate-level resolution of IBV directly from clinical swab samples, including samples that contain multiple isolates.
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