An interaction network of RNA-binding proteins involved in Drosophila oogenesis

Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono

For GO term analysis, the DAVID (Database for Annotation, Visualization and Integrated Discovery) v.6.8 functional annotation tool was used (81, 82) , which adopts Fisher's Exact test to measure the geneenrichment in annotation terms. The following parameters were used: background: Drosophila genome; count threshold (minimum number of genes for that term) of 2; maximum ease score (modified Fisher's Exact P-value) of 0.01. To reduce redundancy in the GO terms, the DAVID output was fed into REVIGO (Reduce + Visualize Gene Ontology) (83) and p-values were used to select and cluster GO terms with a similarity score of 0.7 (medium). The Drosophila database was used to find the GO term sizes. See supplementary table for GO analysis. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.08.899146 doi: bioRxiv preprint