Author: Yao, Changfu; Bora, Stephanie A.; Chen, Peter; Goodridge, Helen S.; Gharib, Sina A.
Title: Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients Cord-id: 53t0tffa Document date: 2021_5_12
ID: 53t0tffa
Snippet: Single-cell RNA sequencing (scRNA-seq) of PBMCs allows in-depth assessment of transcriptional changes in immune cells of COVID-19 patients. However, collecting, processing, and analyzing samples from COVID-19 patients pose many challenges because blood samples may contain infectious virus, identification of immune cell subtypes can be difficult, and biological interpretation of analytical results is complex. To address these issues, we describe a protocol for sample processing, sorting, methanol
Document: Single-cell RNA sequencing (scRNA-seq) of PBMCs allows in-depth assessment of transcriptional changes in immune cells of COVID-19 patients. However, collecting, processing, and analyzing samples from COVID-19 patients pose many challenges because blood samples may contain infectious virus, identification of immune cell subtypes can be difficult, and biological interpretation of analytical results is complex. To address these issues, we describe a protocol for sample processing, sorting, methanol fixation and scRNA-seq analysis of PBMCs from frozen buffy coat samples from COVID-19 patients.
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