Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells Document date: 2020_4_9
ID: fvjh3g7v_4
Snippet: Studies has showed that SARS-CoV-2 S and SARS-CoV S bind with the same receptor, human angiotensin-converting enzyme 2 (hACE2), with similar affinities [7] [8] [9] . Therefore, the studies of SARS-CoV neutralization assays, which based on pseudovirus bearing the spike protein of SARS-CoV, would provide valuable experience and reference for the SARS-CoV-2 assays. In the past years, pseudotyping system based on vesicular stomatitis virus (VSV) was .....
Document: Studies has showed that SARS-CoV-2 S and SARS-CoV S bind with the same receptor, human angiotensin-converting enzyme 2 (hACE2), with similar affinities [7] [8] [9] . Therefore, the studies of SARS-CoV neutralization assays, which based on pseudovirus bearing the spike protein of SARS-CoV, would provide valuable experience and reference for the SARS-CoV-2 assays. In the past years, pseudotyping system based on vesicular stomatitis virus (VSV) was reported to produce pseudotypes incorporating the envelope protein of heterologous risk group-. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.026948 doi: bioRxiv preprint
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