Selected article for: "cc NC ND International license and high throughput"
Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
  • Document date: 2020_4_9
    • ID: fvjh3g7v_7
    Snippet: Recently, Michael Letko et al. used VSVdG-luc bearing SARS-spike chimeras to study the cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses [13] . Jianhui Nie et al. have successfully constructed a pseudovirus neutralization assay for SARS-CoV-2, which consist of pseudotyped VSV bearing the full-length spike protein of SARS-CoV-2 and Huh7 cell [12] . Lentiviral pseudotype bearing the truncated spike protein of SARS-C.....
    Document: Recently, Michael Letko et al. used VSVdG-luc bearing SARS-spike chimeras to study the cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses [13] . Jianhui Nie et al. have successfully constructed a pseudovirus neutralization assay for SARS-CoV-2, which consist of pseudotyped VSV bearing the full-length spike protein of SARS-CoV-2 and Huh7 cell [12] . Lentiviral pseudotype bearing the truncated spike protein of SARS-CoV-2 was also constructed and used to study the virus entry and its immune cross-reactivity with SARS-CoV [6] . Noteworthy, the correlation between pseudovirus system and live . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.026948 doi: bioRxiv preprint virus system hasn't been verified. And the virus package and infection efficiency is also one of the limiting factors for high-throughput in vitro neutralization assay.

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