Author: Renfei Lu; Jian Wang; Min Li; Yaqi Wang; Jia Dong; Weihua Cai
Title: SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge Document date: 2020_3_30
ID: mskkahwi_1
Snippet: For RT-PCR workflow, the primers and probes are from Liferiver Bio-Tech. A25-μlreaction was 116 set up containing 5 μ l of RNA, 19 μ l of reaction buffer provided with the one step RT-PCR 117 system and 1µl enzyme mix. Thermal cycling was performed at 45°C for 10 min for reverse 118 transcription followed by 95°C for 3 min and then 45 cycles of 95 °C for 15 sec, 58 °C for 30 sec 119 in SLAN 96P real time PCR system. 120.....
Document: For RT-PCR workflow, the primers and probes are from Liferiver Bio-Tech. A25-μlreaction was 116 set up containing 5 μ l of RNA, 19 μ l of reaction buffer provided with the one step RT-PCR 117 system and 1µl enzyme mix. Thermal cycling was performed at 45°C for 10 min for reverse 118 transcription followed by 95°C for 3 min and then 45 cycles of 95 °C for 15 sec, 58 °C for 30 sec 119 in SLAN 96P real time PCR system. 120
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