Author: Lu Guo; Xuehan Sun; Xinge Wang; Chen Liang; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Liu Wang; Ruiqi Rachel Wang; Qi Zhou; Wei Li
Title: SARS-CoV-2 detection with CRISPR diagnostics Document date: 2020_4_11
ID: 2una9767_11
Snippet: To validate the specificity of our method for SARS-CoV-2 nucleic acid detection, we tested our protocols against 6 coronaviruses known to cause respiratory diseases (SARS-CoV, MERS-CoV, CoV-HKU1, CoV-229E, CoV-OC43 and CoV-NL63). Consistent with alignment analysis (Supplementary information, Fig. S6a-c) , no cross-reactivity with other endemic human coronavirus were detected (Fig. 1d) . Our results suggested our set of sgRNA and primers showed hi.....
Document: To validate the specificity of our method for SARS-CoV-2 nucleic acid detection, we tested our protocols against 6 coronaviruses known to cause respiratory diseases (SARS-CoV, MERS-CoV, CoV-HKU1, CoV-229E, CoV-OC43 and CoV-NL63). Consistent with alignment analysis (Supplementary information, Fig. S6a-c) , no cross-reactivity with other endemic human coronavirus were detected (Fig. 1d) . Our results suggested our set of sgRNA and primers showed high sensitivity and specificity.
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