Selected article for: "bovine serum and room temperature"
Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein
  • Document date: 2020_3_14
    • ID: 5hpbjkft_31
    Snippet: Vero E6 cells infected with SARS-CoV-2 were harvested and treated with fixative at 4°C . The sections of the cells were prepared through dehydration and embedding. After washing with ultrapure water, the sections were treated with 1% H 2 O 2 for 10 min. Having been blocked with goat serum for 30min, the sections was incubated with corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:200; anti-SARS-CoV-2 Spi.....
    Document: Vero E6 cells infected with SARS-CoV-2 were harvested and treated with fixative at 4°C . The sections of the cells were prepared through dehydration and embedding. After washing with ultrapure water, the sections were treated with 1% H 2 O 2 for 10 min. Having been blocked with goat serum for 30min, the sections was incubated with corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:200; anti-SARS-CoV-2 Spike antibody, 40150-R007, Sino Biological, China, dilution 1:400) for 16 hours at 4º C overnight. Subsequently, the sections were washed with PBS for 5 min, and treated with PBS (containing 1% bovine serum albumin, BSA, pH 8.2) for 7 min. Then the gold colloid-labeling method was used to determine the localization of CD147 and SP for 1h at room temperature, and the sections were consecutively stained with 5% uranium acetate and lead acetate.

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