Author: Cláudia Pereira; Rita M. Reis; José B. Gama; Dhanya K. Cheerambathur; Ana X. Carvalho; Reto Gassmann
Title: Self-assembly of the RZZ complex into filaments drives kinetochore expansion in the absence of microtubule attachment Document date: 2018_3_15
ID: ajkhpw5f_52
Snippet: Measurements of nuclear and cytoplasmic GFP::ROD-1 signal (Fig. 4D, E) were performed after maximum intensity projection of 12 x 1 µm z-stacks, captured every 20 s in embryos developing from the 2-cell to the 8-cell stage. The mean intensity in the nucleus or in the cytoplasm of the same cell was determined, and the mean intensity of a region outside the embryos (camera background) was subtracted......
Document: Measurements of nuclear and cytoplasmic GFP::ROD-1 signal (Fig. 4D, E) were performed after maximum intensity projection of 12 x 1 µm z-stacks, captured every 20 s in embryos developing from the 2-cell to the 8-cell stage. The mean intensity in the nucleus or in the cytoplasm of the same cell was determined, and the mean intensity of a region outside the embryos (camera background) was subtracted.
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