Sensitive one-step isothermal detection of pathogen-derived RNAs

Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee

Lastly, we applied the orthogonal dual detection to the SARS-CoV-2, which has many 257 related viruses with high sequence homology. Simultaneous detection of multiple target sites 258 along its genome would enable specific discrimination of this emerging pathogen from 259 others. In addition to the previously demonstrated SARS-CoV-2 probe pair (Fig. 4f) CoV-2 RNA, thereby exhibiting higher fluorescence intensity compared to that of the related 267 viral RNA sequences (Fig. 7b) . We then tested the orthogonal dual detection of two target 268 regions using the SARS-CoV-2-MG1 and SARS-CoV-2-BR2 probe pairs. Dual SENSR 269 assay effectively detected the target RNA and maintained the specificity of each probe pair 270 (Fig. 7c) . Therefore, the dual SENSR assay could be used to assist diagnostic decision 271 making by providing two detection results that can complement each other. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.