Author: Tan, Xin-yu; Fan, Zheng; Wang, Hua-jin; Shi, Lei; Yin, Bin; Ni, An-ping; Qin, Chuan; Zou, Ke; Shen, Yan; Yuan, Jian-gang; Qiang, Bo-qin; Peng, Xiao-zhong
Title: [Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein]. Cord-id: c9of3hvl Document date: 2003_1_1
ID: c9of3hvl
Snippet: OBJECTIVE To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain. METHODS According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA. RESULTS Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was ob
Document: OBJECTIVE To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain. METHODS According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA. RESULTS Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained. CONCLUSIONS The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.
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