Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs Document date: 2020_3_9
ID: 1uqjmeic_41
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is Inhibitor (20 U), 2 μL T7 RNA polymerase (50 U), and 8.7 μL RNase-free water was 355 incubated at 37 °C for 16 h. The resulting reaction products were treated with 1 μL of DNase 356 I (RNase-free) for 1 h at 37 °C. The transcript was purified using the Riboclear TM (plus!) 357 RNA kit (GeneAll, Seoul, Republic of Korea) and quantified using the absorbance at 260 nm. 358.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is Inhibitor (20 U), 2 μL T7 RNA polymerase (50 U), and 8.7 μL RNase-free water was 355 incubated at 37 °C for 16 h. The resulting reaction products were treated with 1 μL of DNase 356 I (RNase-free) for 1 h at 37 °C. The transcript was purified using the Riboclear TM (plus!) 357 RNA kit (GeneAll, Seoul, Republic of Korea) and quantified using the absorbance at 260 nm. 358
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