Selected article for: "amplified product and PCR product"
Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs
  • Document date: 2020_3_9
    • ID: 1uqjmeic_43
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03.05.20031971 doi: medRxiv preprint 16 Clara, CA, USA) using an RNA 6000 nano kit (Agilent Technologies) following the 361 manufacturer's direction. All primers are listed in Supplementary Table 4 RNase-free water. The mixture was heated to 95 °C for 3 min, then slowly cooled to room 377 temperature. This was followed by the addition of 1 μL .....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03.05.20031971 doi: medRxiv preprint 16 Clara, CA, USA) using an RNA 6000 nano kit (Agilent Technologies) following the 361 manufacturer's direction. All primers are listed in Supplementary Table 4 RNase-free water. The mixture was heated to 95 °C for 3 min, then slowly cooled to room 377 temperature. This was followed by the addition of 1 μL 10 SplintR buffer and 0.5 μL of 378 SplintR ligase (25 U), and incubation of the mixture at 37 °C for 30 min. The reaction was 379 terminated by heating at 95 °C for 10 min. The ligated product was amplified through PCR 380 reaction with LigChk_F and LigChk_R primers (Supplementary Table 4 ). The PCR products 381

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