Sensitive one-step isothermal detection of pathogen-derived RNAs

Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee

The copyright holder for this preprint (which was not peer-reviewed) is samples. The detection limit of SENSR is as low as 2 CFU per 100 µL reaction. c, Detection 591 of bacterial cell diluted in human serum as a proxy clinical sample. Bacteria-contained human 592 serum was thermally lysed and subjected to the SENSR reaction. d, An obvious distinction in 593 the fluorescence intensity between MRSA-and MSSA-contained human serum was 594 observed. The detection limit of SENSR is as low as 2 CFU per 100 µL reaction. All tests are 595 four experimental replicas (two-tailed student's test; * P < 0.05, ** P < 0.01, *** P < 0.001, 596 **** P < 0.0001; bars represent mean ± s.d). 597 All rights reserved. No reuse allowed without permission. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.