Author: Sanekata, Takeshi; Kishimoto, Eiichi; Sato, Kunihiko; Honma, Hitoshi; Otsuki, Koichi; Tsubokura, Misao
Title: Detection of porcine rotavirus in stools by a latex agglutination test Cord-id: 6idcnt1i Document date: 2002_11_5
ID: 6idcnt1i
Snippet: We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOSU). The agglutination of LA-antiOSU with OSU on a glass slide evident macroscopically within 2 min. The sensitivity of this latex agglutination (LA) test was four times higher than that of the electro
Document: We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOSU). The agglutination of LA-antiOSU with OSU on a glass slide evident macroscopically within 2 min. The sensitivity of this latex agglutination (LA) test was four times higher than that of the electron microscope method. The LA test is available for the rapid diagnosis of porcine rotavirus infections.
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