Selected article for: "flow rate and formic acid"
Author: Lucia Grenga; Fabrice Gallais; Olivier Pible; Jean-Charles Gaillard; Duarte Gouveia; Hélène Batina; Niza Bazaline; Sylvie Ruat; Karen Culotta; Guylaine Miotello; Stéphanie Debroas; Marie-Anne Roncato; Gérard Steinmetz; Charlotte Foissard; Anne Desplan; Béatrice Alpha-Bazin; Christine Almunia; Fabienne Gas; Laurent Bellanger; Jean Armengaud
Title: Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines
  • Document date: 2020_4_17
    • ID: kd02dehk_12
    Snippet: Peptides were identified using an ultimate 3000 nano-LC system (Thermo Fisher Scientific) coupled with a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). Peptides were desalted on a reversed-phase PepMap 100 C18 μ-precolumn (5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Fisher Scientific) before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate o.....
    Document: Peptides were identified using an ultimate 3000 nano-LC system (Thermo Fisher Scientific) coupled with a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). Peptides were desalted on a reversed-phase PepMap 100 C18 μ-precolumn (5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Fisher Scientific) before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate of 0.2 μL per min using a 120 min gradient comprising 100 min from 4% to 25% of solvent B and 20 min from 25% to 40% of solvent B (solvent A consisted in 0.1% formic acid in water, solvent B was 80% acetonitrile, 0.1% formic acid in water). The mass spectrometer was operated in Top20 mode. Full MS were acquired from 350 to 1,500 m/z and the 20 most abundant precursor ions were selected for fragmentation with 10 s dynamic exclusion time. Ions with 2+ and 3+ charge were selected for MS/MS analysis. Secondary ions were isolated with a window of 2.0 m/z. author/funder. All rights reserved. No reuse allowed without permission.

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