Altered polarization of PAR-2 signaling during airway epithelial remodeling

Ryan M. Carey; Jenna R. Freund; Benjamin M. Hariri; Nithin D. Adappa; James N. Palmer; Robert J. Lee

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.09.900555 doi: bioRxiv preprint remove pituitary tumors). Tissue was mounted, imaged by confocal microscopy, and stimulated apically with PAR-2 agonists. Polyp tissue exhibited apical responses to 2FLI, trypsin, or Der p 3, but not thrombin (Fig 6A-B) . Control uninflamed middle turbinate tissue did not (Fig 6C) . Data are summarized in (Fig 6D) . Immunofluorescence revealed that PAR-2 was nonetheless basolaterally expressed in ciliated cells from nasal polyp (Fig 6E) , as we previously reported for non-polyp tissue. 8 Moreover, the calcium responses to PAR-2 stimulation were no enhanced in isolated polyp vs turbinate ciliated cells (Fig 6F) . We thus hypothesized that apical PAR-2 responses do not originate from within the ciliated cells themselves.