Author: Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger
Title: Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX Document date: 2018_5_13
ID: 298cbr1x_26
Snippet: The fact that purified SOX endonucleolytically cleaved LIMD1 54 at the precise site observed in SOX-expressing cells demonstrates that cleavage site selection on an mRNA is not mediated by a cellular cofactor. Instead, targeting at particular RNA motifs is strongly influenced by the strength of SOX binding. Our observation that the P176S and F179A SOX mutants display significant RNA binding defects indicates that their failure to cleave mRNAs in .....
Document: The fact that purified SOX endonucleolytically cleaved LIMD1 54 at the precise site observed in SOX-expressing cells demonstrates that cleavage site selection on an mRNA is not mediated by a cellular cofactor. Instead, targeting at particular RNA motifs is strongly influenced by the strength of SOX binding. Our observation that the P176S and F179A SOX mutants display significant RNA binding defects indicates that their failure to cleave mRNAs in cells is due to an inability to efficiently bind the targeting motif.
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