Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX

Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger

Here, we revealed that in addition to the requirement for an unpaired loop at the cleavage site, additional upstream RNA sequences increase the affinity of SOX for individual targets, thereby controlling cleavage efficiency.