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Author: Ki Hyun Kim; Jinhee Kim; Meehyun Ko; June Young Chun; Hyori Kim; Seungtaek Kim; Ji-Young Min; Wan Beom Park; Myoung-don Oh; Junho Chung
Title: An anti-Gn glycoprotein antibody from a convalescent patient potently inhibits the infection of severe fever with thrombocytopenia syndrome virus
  • Document date: 2018_10_3
    • ID: 3o4mw305_4
    Snippet: 213 Also, mutation of the flanking residues of cystine, corresponding to G351, L354, E355, I357, . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/434530 doi: bioRxiv preprint 214 T374, and V375 also reduced the reactivity by over 60% . In the cases of mutation residues 215 which are distant from cysteine residues, m.....
    Document: 213 Also, mutation of the flanking residues of cystine, corresponding to G351, L354, E355, I357, . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/434530 doi: bioRxiv preprint 214 T374, and V375 also reduced the reactivity by over 60% . In the cases of mutation residues 215 which are distant from cysteine residues, mutation of G360, V361, R362, L363, T365, L370, 216 G387, and K389 residues reduced the relative reactivity by more than 80%. The other 217 residues had minor effects on reactivity. Overall, Ab10 binding to Gn was predicted to be 218 affected by 25 amino acid residues within domain II and the stem region of SFTSV Gn 327 For alanine-scanning mutagenesis, SFTSV Gn glycoprotein with amino acid residues (315-328 389) substituted with alanine were produced by cloning synthesized DNA fragments 329 (Integrated DNA Technologies) into modified pCEP4 vector, as described above.

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