Author: Wanchao Yin; Chunyou Mao; Xiaodong Luan; Dan-Dan Shen; Qingya Shen; Haixia Su; Xiaoxi Wang; Fulai Zhou; Wenfeng Zhao; Minqi Gao; Shenghai Chang; Yuan-Chao Xie; Guanghui Tian; He-Wei Jiang; Sheng-Ce Tao; Jingshan Shen; Yi Jiang; Hualiang Jiang; Yechun Xu; Shuyang Zhang; Yan Zhang; H. Eric Xu
Title: Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir Document date: 2020_4_9
ID: 7v7pzclb_19
Snippet: In addition, the gene of nsp7 and nsp8 were respectively cloned into a modified pET-32a(+) vector containing a 5' ATG starting sequence and C-terminal His8 tag for expression in E. coli. Plasmids were transformed into BL21(DE3)( Invitrogen). Bacterial cultures were grown to an OD600 of 0.6 at 37 °C, and then the expression was induced with a final concentration of 0.1 mM of isopropyl β-D-1-thiogalactopyranoside (IPTG) and the growth temperature.....
Document: In addition, the gene of nsp7 and nsp8 were respectively cloned into a modified pET-32a(+) vector containing a 5' ATG starting sequence and C-terminal His8 tag for expression in E. coli. Plasmids were transformed into BL21(DE3)( Invitrogen). Bacterial cultures were grown to an OD600 of 0.6 at 37 °C, and then the expression was induced with a final concentration of 0.1 mM of isopropyl β-D-1-thiogalactopyranoside (IPTG) and the growth temperature was reduced to 16 °C for 18-20 h. The bacterial cultures were pelleted and stored at − 80 °C until use.
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