Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome

Ramasubramanian Sundaramoorthy; Amanda L. Hughes; Hassane El-Mkami; David Norman; Tom Owen-Hughes

The prepared grids are initially checked for its ice quality and the particle distribution using a JEOL 2010 microscope with side-entry cryo-holder operated at 200 keV and equipped with a gatan 4k × 4k CCD camera. Cryogrids were then stored in liquid nitrogen and dry-shipped to respective centre for data collection. For the 1:1 complex the data was acquired on a FEI Titan Krios transmission electron microscope (TEM) operated at 300 keV, equipped with a K2 summit direct detector (Gatan). Automated data . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/290874 doi: bioRxiv preprint acquisition was carried out using FEI EPU software at a nominal magnification of 105,000×.