Author: Adriana Larrea-Sarmiento; Anne M. Alvarez; James P. Stack; Mohammad Arif
Title: Synergetic effect of non-complementary 5’ AT-rich sequences on the development of a multiplex TaqMan real-time PCR for specific and robust detection of Clavibacter michiganensis and C. michiganensis subsp. nebraskensis Document date: 2019_3_11
ID: iniz1rjk_12_1
Snippet: wed) is the author/funder. It . https://doi.org/10.1101/566281 doi: bioRxiv preprint cycling conditions consisted of a PCR initial activation step at 95°C for 5 min, following by rapid 40 cycles of denaturation at 95°C for 15 sec and annealing/extension at 60°C for 15 sec. Cycle threshold (C T ) values were calculated and analyzed with Rotor-Gene® Q series software (Qiagen)......
Document: wed) is the author/funder. It . https://doi.org/10.1101/566281 doi: bioRxiv preprint cycling conditions consisted of a PCR initial activation step at 95°C for 5 min, following by rapid 40 cycles of denaturation at 95°C for 15 sec and annealing/extension at 60°C for 15 sec. Cycle threshold (C T ) values were calculated and analyzed with Rotor-Gene® Q series software (Qiagen).
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