Platelet-rich plasma protects human melanocytes from oxidative stress and ameliorates melanogenesis induced by UVB irradiation.

Ma, Yongshi; Xuan, Min; Dong, Yunqing; Wang, Hong; Huang, Jianfang; Cheng, Biao; Cui, Xiao

To investigate the role of Platelet-rich plasma(PRP) from different sources in alleviating oxidative stress and ameliorating melanogenesis in UVB-irradiated PIG1 cells. PIG1 cells were irradiated with 80 mJ/cm2 UVB prior to 1% PRP application and the following experiments were taken: The viability of UVB-irradiated PIG1 cells, cellular malondialdehyde (MDA) and reactive oxygen species (ROS)content, and activities of anti-oxidant enzymes. Western blotting was utilized to detect the expression level of proteins associated with melanin synthesis, apoptosis and DNA lesions. We found that PRP intervention promoted cell proliferation, reduced MDA and ROS content, increased the activities of series of anti-oxidant enzymes and alleviated DNA damages in UVB-damaged PIG1 cells. Of notes, PRP treatment inhibited UVB-induced melanogenesis via PI3K/Akt/GSK3β signal pathway. Therefore, we suppose PRP treatment exerts protective role through their anti-oxidation effect on UVB-damaged PIG1 cells and hinders melanogenesis induced by UVB irradiation.