Author: Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf
Title: The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19 Document date: 2020_2_11
ID: 7vrm081c_42
Snippet: construct. An amino acid can be encoded by different codons in different organisms, which is known as codon bias. Codon adaptation predicts the best codon for a specific amino acid that . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint should work effectively and efficiently in a.....
Document: construct. An amino acid can be encoded by different codons in different organisms, which is known as codon bias. Codon adaptation predicts the best codon for a specific amino acid that . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint should work effectively and efficiently in a specific organism. The best predicted vaccine was used for codon adaptation by the Java Codon Adaptation Tool or JCat server (http://www.jcat.de/). The server ensures the maximal expression of protein in a target organism. Eukaryotic E. coli strain K12 was selected at the JCat server and rho-independent transcription terminators, prokaryotic ribosome binding sites and SgrA1 and SphI cleavage sites of restriction enzymes, were avoided.
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