The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19

Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf

After the successful docking study, the vaccine construction was performed. The linkers were used to connect the T-cell and B-cell epitopes among themselves and also with the adjuvant sequences as well as the PADRE sequence. The vaccines, with three different adjuvants, were constructed and designated as: CV-1, CV-2 and CV-3. Since all the three vaccines were found to be antigenic, they should be able to induce good immune response. Moreover, all of them were possibly nonallergenic, they should not be able to cause any allergenic reaction within the body as per in silico The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint antigen. Moreover, the conventional approach of vaccine development have raised many safety concerns in many pre-clinical and clinical trials. The subunit vaccines like the vaccines predicted in the study could overcome such difficulties [196] - [200] . Finally, this study recommends CV-1 as the best vaccine to be an effective worldwide treatment based on the strategies employed in the study to be triggered against SARS-CoV-2 infection. However, further in vivo and in vitro experiments are suggested to strengthen the findings of this study.