Author: Conzentino, Marcelo S.; Santos, Tatielle P.C.; Selim, Khaled A.; Wagner, Berenike; Alford, Janette T.; Deobald, Nelli; Paula, Nigela M.; Rego, Fabiane G.M.; Zanette, Dalila L.; Aoki, Mateus N.; Nardin, Jeanine M.; Huergo, Maria C.C.; Reis, Rodrigo A.; Huergo, Luciano F.
Title: Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads Cord-id: 5k3732kj Document date: 2021_10_15
ID: 5k3732kj
Snippet: To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni(2+) magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and i
Document: To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni(2+) magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
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