Author: José L. Martínez; Francesca Arnoldi; Elisabeth M. Schraner; Catherine Eichwald; Daniela Silva-Ayala; Eunjoo Lee; Elizabeth Sztul; Óscar R. Burrone; Susana López; Carlos F. Arias
Title: The guanine nucleotide exchange factor GBF1 participates in rotavirus replication Document date: 2019_4_29
ID: jkjkkjrf_19
Snippet: To confirm that the alterations in the electrophoretic mobility of VP7 and NSP4 were due to 217 the inhibition of GBF1, MA104 cells were transfected with the siRNA to GBF1 to silence its 218 expression, and infected with RRV; at 7.5 hpi the cells were pulse-labeled with 35 S-Met as 219 described above. As observed when the pharmacological inhibitors were used, knocking 220 down the expression of GBF1 also caused changes in the electrophoretic mob.....
Document: To confirm that the alterations in the electrophoretic mobility of VP7 and NSP4 were due to 217 the inhibition of GBF1, MA104 cells were transfected with the siRNA to GBF1 to silence its 218 expression, and infected with RRV; at 7.5 hpi the cells were pulse-labeled with 35 S-Met as 219 described above. As observed when the pharmacological inhibitors were used, knocking 220 down the expression of GBF1 also caused changes in the electrophoretic mobility of VP7 221 and NSP4 ( Fig 5C) ; in this case, the change was partial, most likely due to incomplete GBF1 222 knock down in all cells. To our knowledge GBF1 is the first identified regulatory cellular 223 factor that affects the mobility of VP7 and NSP4.
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