The Infectious Bronchitis Virus Coronavirus Envelope Protein Alters Golgi pH to Protect Spike Protein and Promote Release of Infectious Virus

Jason W. Westerbeck; Carolyn E. Machamer

Bands were detected with donkey anti-rabbit IgG-680 (LI-COR) diluted in TBS/Tw, and imaged using the LI-COR Odyssey CLx. Quantification was performed in Image Studio (LI-COR), and each IBV S fragment was expressed as the percent of the total S for both the input and surface fractions. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/440628 doi: bioRxiv preprint labeled with rabbit anti-GFP and mouse anti-M2, followed by Alexa 488 anti-rabbit IgG and Alexa 546 anti-mouse IgG, and Hoescht stain. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/440628 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/440628 doi: bioRxiv preprint